基于荧光猝灭原理的光纤化学传感器研究 Ⅰ、基于荧光猝灭原理的光纤化学传感器的工作原理和研制方法

根据卤素离子、重金属离子、氧分子和芳香硝基化合物等物质能减低某些荧光试剂的荧光强度或缩短荧光寿命的性质,用不同的荧光分子探针,加入适当的聚合物和稳定剂制成透明、有弹性的薄膜。装置在光导纤维的端部或采用荧光试杯法,作为光学传感器用于测定一系列无机和有机药物。实验研究证明,这类传感器有较好的灵敏度、可逆性和快速响应等优点,符合Stern-Volmer公式。可以连续在线分析静止或流动的样品,在药物分析和环境监测等领域有广阔的应用前景。作者采用新的聚合物和增塑荆材料研制成具有优良性能的芘丁酸(PBA)敏感膜,并为研制其它系列敏感膜准备了条件。     

51例冠心病患者的动态心电图分析

对584例24h动态心电图中有一过性心肌缺血的51例冠心病患者进行分析,发现51例中有144次缺血发作,其中无症状性发作114次(占79.2％)、症状性发作30次(占20.8％)。无症状性发作明显多于症状性发作,尤其是心梗后患者,且其无症状性发作的频度及持续时间与非心梗患者组相比,有显著差异(p<0.05)。说明无症状性心肌缺血有重要临床意义。     

The objective clinical assessment of the quality of the anterior segment by means of the Oqual

The Oqual is a simple new device attachable to the (Haag-Streit or Topcon) slit-lamp. It provides objective numerical assessments of the optical quality of the anterior segment. In the present study, patients attending Outpatients Clinics at Moorfields Eye Hospital were divided into those who suffered from lenticular symptoms and those who did not. Their monocular visual acuity was measured routinely by means of a Snellen chart, and thereafter their eyes were examined with the Oqual. Results are given for the correlation between the two measures overall (R² = 47.35%), and for inter-observer performance (r = 0.8878). Earlier conclusions on the effects of smoking habits are supported.     

体部X线动态三维适形放疗的固定与摆位技术

本文简要地介绍了体部肿瘤动态三维适形放疗的体位固定、治疗方法和摆位技术。     

152例青年学生晕厥患者动态心电图分析

目的:评价动态心电图在青年晕厥患者中的诊断价值。方法:在152例青年晕厥患者中,进行了动态心电图和心电图检查。结果:对窦性心动过速,各种传导阻滞,Q-T间期延长的检出率与普通心电图差异不大,而对窦性静止,快-慢综合征,阵发性室上性心动过速,阵发性室性心动过速动态心电图明显优于普通心电图(P<0.01)。结论:动态心电图为诊断心源性晕厥可提供依据。     

房性早搏诱发阵发性心房颤动的动态心电图分析

目的探讨房性早搏(简称房早)与阵发性心房颤动(简称房颤)的关系。方法选择经动态心电图诊断为阵发性心房颤动的患者64例。统计其24 h房性早搏总数,房颤发作的阵数,诱发和未诱发房颤的房性早搏的联律间期及房早前周期等。结果经动态心电图记录阵发性心房颤动患者诱发房颤的房早均呈频发房性早PonT现象;诱发房颤的房早联律间期(346.7±38.5 ms)比未诱发房颤的房早联律间期(412.3±40.2 ms)明显提前(P<0.01);诱发房颤的房早前周期(836±115 ms)比未诱发房颤的房早前周期(733±118 ms)明显延长(P<0.01)。结论阵发性心房颤动由房性早搏始动与房性早搏的联律间期及房早前周期有关。动态心电图是诊断阵发性心房颤动可靠、高效、无创、重复性好的检查手段。     

OSA microarray reader instrumentation and its applications for registration of on-chip real-time reactions

New applications of microarray technology requires new approaches to microarray reader development. Miniaturization of the biochips, necessity of massive parallel microarray-based analysis of various biological samples as well as increasing application of different real-time on-chip reactions for research and diagnostics demand improvement of microarray reader instrumentation. We represent the optical scanning array (OSA) reader system, allowing fast capture of high-resolution multifluorescence images and equipped with thermocontrolled hybridization chamber which is required for registration of a wide range of on-chip real-time processes. We demonstrate the application of OSA microarray reader for optimisation of hybridization and washing conditions for microarray-based detection of Q493X mutation of CFTR gene. It was shown that detailed analysis of on-chip thermodynamic and kinetic behaviour of oligonucleotide/oligonucleotide and ssPCR/oligonucleotide duplexes is very helpful for optimisation of experimental procedure.     

Optimal dynamic irrigation schemes

Optimal control methods are employed to derive irrigation management schemes accounting both for the dynamic response of the biomass yield to soil moisture and for the cost of irrigation water. Moisture dynamics depend on the irrigation rate and on the current biomass and moisture states. We find that the optimal irrigation policy has turnpike characteristics: soil moisture in the root zone should be brought to some optimal target level as rapidly as possible and kept at that level until some time prior to harvest, when irrigation should be ceased. The target moisture level and the stopping time vary across crops, soil types, climatic conditions and economic (price) factors, but the turnpike structure of the optimal irrigation policy persists under general circumstances. An empirical example demonstrates that the optimal scheme significantly outperforms the policy of yield maximization in actual practice. Copyright © 2004 John Wiley & Sons, Ltd.     

Spiral MR myocardial tagging.

In the present study, complementary spatial modulation of magnetization (CSPAMM) myocardial tagging was extended with an interleaved spiral imaging sequence. The use of a spiral sequence enables the acquisition of grid-tagged images with a tagline distance as low as 4 mm in a single breath-hold. Alternatively, a high temporal resolution of 77 frames per second was obtained with 8-mm grid spacing. Ten healthy adult subjects were studied. With this new approach, high-quality images can be obtained and the tags persist throughout the entire cardiac cycle.